Effect of permissive and non-permissive temperatures on responses of temperature sensitive (ts) SV40 transformed human vascular smooth muscle cells
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چکیده
منابع مشابه
cAMP production by piglet cerebral vascular smooth muscle cells: pHo, pHi, and permissive action of PGI2.
In newborn pig pial arterioles and cocultures of cerebral microvascular endothelial and smooth muscle cells, hypercapnia increases cAMP. In the intact cerebral circulation, both the increase in cAMP and the accompanying vasodilation require the presence of PGI2. Using piglet cerebral microvascular smooth muscle in primary culture, we addressed the hypothesis that, in the presence of PGI2, hyper...
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چکیده ندارد.
15 صفحه اولHuman placental cells transformed with temperature-sensitive simian virus 40 are immortalized and mimic the phenotype of invasive cytotrophoblasts at both permissive and nonpermissive temperatures.
Establishment of the human placenta is essential for subsequent development of the embryo. Previous studies from our laboratories have demonstrated that chorionic villus cytotrophoblast stem cells undergo a stepwise differentiation process in vivo that results in their ability to invade the uterine wall. This process can be mimicked by isolated primary first-trimester cytotrophoblasts in vitro....
متن کاملConditional differentiation of heart- and smooth muscle-derived cells transformed by a temperature-sensitive mutant of SV40 T antigen.
To create muscle cell lines that conditionally differentiate in vitro we introduced a temperature-sensitive SV40 T antigen by retroviral infection into rat aortic smooth muscle cells (SMCs) and neonatal heart-derived cells. After G418 selection cell lines isolated were characterized at permissive (33 degrees C) and non-permissive (39 degrees C) temperatures. [3H]Thymidine uptake showed tht prog...
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The role of oxidized high-density lipoprotein (oxHDL) and the protective effects of adiponectin in terms of vascular calcification is not well established. This study was conducted to investigate the effects of oxHDL with regards to inflammation and vascular calcification and to determine the protective role of adiponectin in attenuating the detrimental effects of oxHDL. Cell viability, mineral...
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ژورنال
عنوان ژورنال: American Journal of Hypertension
سال: 2001
ISSN: 0895-7061
DOI: 10.1016/s0895-7061(01)01576-x